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The microbial community transcriptome analyses revealed that the identification of indigenous gene- and taxon-specific patterns, and the identification of key metabolic functions are feasible.
Genetic monitoring of archived samples collected over time may provide a tool for quantifying the potential for hatchery programs to change the diversity and structure of indigenous gene pools and hence, guide subsequent management plans (Schwartz et al. 2007; Allendorf et al. 2008; Van Doornik et al. 2011).
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Additional studies are required to estimate relative duplication rates of foreign and indigenous genes, rates of mutations, and paralog silencing in this group of organisms.
RIN analysis and qRT-PCR of spiked-in and indigenous genes revealed that RNA Later preserved mRNA integrity most efficiently, while samples conserved in RNA Protect showed substantial mRNA decay.
These findings suggest that non-indigenous genes may have been introduced into Puerto Ayacucho.
The fact that DU is the only location on the east coast with these characteristics suggests that the introduction of non-indigenous genes from the western range detected only here is a consequence of human-mediated larval transport.
Similar results were obtained using the indigenous actin gene as the reference gene (data not shown).
The ratios of the inserted copy-numbers of the five genes in the constructed KR5 isolates relative to the indigenous alg9 gene were approximately 1.7 (kan), 1.2 (egIII), 2.3 (cbhI), 0.9 (gfp) and 6.2 (npabgs).
In addition to these three lines where the indigenous Lmna gene was modified, transgenic lines were derived in which Progerin was expressed as a transgene.
The mRNA levels of the seven genes were 46.0 (kanMX) : 8.7 (egIII) : 1.4 (cbhI) : 6.4 (eglA) : 8.9 (cbhII) : 9.2 (cdtI-gfp) : 53.6 (npabgs) relative to the indigenous alg9 gene in KR7.
The observed inserted copy-number ratios of the seven genes were approximately 2 (kanMX) : 2 (egIII) : 6 (cbhI) : 4 (eglA) : 2 (cbhII) : 2 (cdtI-gfp) : 7 (npabgs) relative to the indigenous alg9 gene (2 copies) in KR7.
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