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Cleaned fragments were sequenced from both ends using the di-deoxy chain terminator method [34], with V3.1 Bigdye terminator chemistry [35].
Purified PCR products were first directly sequenced from both ends using the forward and reverse primer.
Inserts were sequenced from both ends using the standard Sanger method.
BAC clones were sequenced from both ends using the primers T7 (5'-TAATACGACTCACTATAGGG-3') and SP6 (5'-ATTTAGGTGACACTATAG-3').
The PCR products were purified (QIAquick PCR Purification Kit, Qiagen, CA) and sequenced from both ends using the PCR primers.
The full-length sequences were finished by trimming the vector-derived sequences from both ends using the cross_match program.
Similar(44)
After tying a knot in both ends, use the bound soap scrap bag as an exfoliating body scrubber.
The 8 cDNA libraries were pooled and simultaneously sequenced from both 5' and 3' ends using the Illumina MiSeq ®; System platform according to the manufacturer's instructions.
The cDNA library was sequenced from both 5′ and 3′ ends using the Illumina HiSeq 2000 platform by following the manufacturer's instructions.
The cDNA library was sequenced from both 5′ and 3′ ends using the Illumina HiSeq™ 2000 platform according to the manufacturer's instructions.
A conventional approach is to determine the identities of the clones by sequencing from one or both ends using primers specific to the vector sequence [ 5- 7].
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