Sentence examples for both directions using the from inspiring English sources

Purified PCR products were direct sequenced in both directions using the appropriate PCR primers (ASGPB, University of Hawaìi at Manoa).

The amplicons of this region were sequenced in both directions using the appropriate primers.

Amplified DNA from PCR was directly sequenced in both directions using the BigDye terminators Ready Reaction Kit (PE Applied Biosystems, Foster City, California) on an ABI Prism automated DNA sequencer (model 377, version 2.1.1; Applied Biosystems Warrington, United Kingdom).

PCR products were sequenced directly in both directions using the same primers.

Amplified DNA samples were directly sequenced in both directions using the CP1F and CP2R primers.

PCR products were purified and sequenced by Macrogen Inc. (Seoul, Korea) in both directions using the PCR primers.

Sequencing was carried out in both directions using the BigDye Terminator cycle sequencing kit (Perkin-Elmer) on an automated ABI PRISM model 377 machine (Applied Biosystems).

Sequencing of each PCR reaction was performed in both directions using the M13 Forward and M13 Reverse primers provided with the TOPO TA cloning kit.

Sequencing was performed from both directions using the Applied Biosystems 3700 or 3730 automated DNA sequencers (Applied Biosystems Inc) according to the manufacturer's recommendation.

Sequencing reactions were performed in both directions using the Big Dye terminator technology (version 3.2; Applied Biosystems) and products were detected with an ABI 3130 Genetic Analyzer (Applied Biosystems).

The amplified DNA from all isolates was cycle sequenced in both directions using the BigDye Terminators Reaction Kit v. 3.1 (Applied Biosystems, Foster City, CA) on an ABI automated DNA sequencer (3730 XL DNA Analyzer).