Sentence examples for both strands using the from inspiring English sources

All PCR products were purified and sequenced on both strands using the same primers.

PCR products were directly sequenced on both strands using the same AFLP primers.

Amplicons were then purified and sequenced on both strands using the BigDye Terminator v3.1 kit (Applied Biosystems, conditions: 96°C, 5mn, 1 cycle, 96°C, 10 sec, 60°C 5 sec, 60°C 4mn, 25 cycles) and analysed on a 3130 Genetic Analyser (Applied Biosystems).

The product was directly sequenced in both strands using the PCR oligonucleotides by STAB VIDA (Portugal).

PCR products were sequenced on both strands using the BigDye kit (Applied Biosystems).

PCR products were purified onto Millipore PCR clean-up plates and directly sequenced on both strands using the BigDye v1.1 kit (Life Technologies).

Successfully amplified products were sequenced for both complementary strands using the DYEnamic™ ET dye terminator kit (Amersham Biosciences), and samples were run on MegaBACE 1000 (Amersham Biosciences) according to the manufacturer protocol.

Sequencing of PCR-amplified fragments was carried out on both DNA strands using the Ampli Taq Polymerase FS, the Big Dye Terminator 1.1 sequencing kit (Applera), and a ABI PRISM 3100 sequencer (Perkin Elmer).

Purified PCR products were directly sequenced on both strands using either the PCR primers or internal primers.

The PCR products were purified with ExoSAP (USB, USA) and sequenced for both forward and reverse strands using the BigDye Terminator chemistry version 3 kit according to the standard protocol (Applied Biosystems, CA).

All the PCR amplification products from LM50 were sequenced (both strands) using conserved primers, the BigDye Terminator Cycle Sequencing kit, version 3.1 (Applied Biosystems, Beijing, China), and a Li-Cor 4300 genetic analyzer (Li-Cor Biosciences, Lincoln, Nebraska, USA).