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Photoshop CS4 (Adobe, San Jose, CA) and a proprietary script were used for automated quantification of migration 5 × 10 SCC-9 cells were allowed to migrate for 6 hrs (chemotaxis) or 17 hrs (intrinsic motility).
Importantly, due to their high motility, movies of migrating MEC1 cells are easily accessible to the automated computer-based quantification of migration parameters of individual cells.
The quantification of migration was further analyzed by counting in a double-blind manner (i.e., by two independent associates).
(C ) E19.5 quantification of migration in control and Gsk3 over-expressing neurons using 8 bin analysis as previously described.
Quantification of migration directionality relates the linear distance between the start and end points (D) to the total distance (T) traveled.
(D) Representative images of cell migration by Vector and WT BST-2 expressing cells and Image J quantification of migration events (bars).
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Quantification of migrated cells from cell migration experiments are generally limited to objective powers ranging from 5 × to 200 × [ 14] and thus require 3 [ 15] to 10 [ 16] fields of view in an effort to capture a representative sample of the membrane.
The right panel of Figure 6B depicts quantification of cell migration relative to cell migration in the absence of EPO.
Quantification of the migration rate in scratch assay showed significantly increased migration with TGF- β2 treatment, in parallel to spheroid assay results.
Insert membranes were isolated following migration and stained with DAPI to allow for quantification of migrated cells (total magnification, 100×).
(iii) Combining (i) and (ii) allowed quantification of cell migration independent of cell division.
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