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The assay of migration inhibition was similar to the migration procedure described above, except that MGC803 cells were seeded onto the top chamber of each Transwell and different concentrations of normal rabbit IgG, rabbit anti-GST or rabbit anti-p37 antibody were added simultaneously.
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To unravel the molecular mechanisms underlining the increased migration of MABs in the absence of JAM-A, we used an in vitro assay of MAB migration through cultured endothelial cells.
The role of DDAH I on vessel development was examined further using an in vitro assay of endothelial migration.
An assay of transepithelial migration was performed as above, but using unlabeled T cells, and with blocking mAbs as appropriate.
To investigate whether immobilised vitronectin could also stimulate migratory responses in TIL, we used an assay of haptotaxis (migration on an immobilised substrate).
The design concept of this system is the same as that used for the assay of transendothelial migration except that the monolayer of HMEC-1 is replaced with a uniform layer of BD Matrigel Basement Membrane Matrix BD Biosciencess).
We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient.
Also, the lack of quantitative outputs from this type of assay and lack of migration observation make analysis less clear.
The rounded "amoeboid" form of movement seen in vivo is not revealed by assays of cell migration on rigid 2D substrates, or transwell assays where cells have to migrate through pores coated with a thin layer of extra-cellular matrix [3], [4].
Assays of cell migration were performed by using Transwell chambers (10 mm diameter, 8 µm pores, Costar Corp).
However, carcinoma cells responded well to serum stimulation in assays of cell migration and growth.
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CEO of Professional Science Editing for Scientists @ prosciediting.com