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Exact(44)
7) Homologous interacting and thus interchangeable protein sequences may also be shared between the carboxyl terminus of RAG-1 and the amino terminus of RAG-2 as well as the herpes DBP as a consequence of the original insertion event [69] Obviously, it will not be possible to revisit the origins of the acquired immune system except through empirically testable hypotheses.
In our first case, the dislocation occurred 5 years after its original insertion following a twisting injury to the knee.
Further improvement of the original insertion scheme led to the set up of a large number of whole-cell biosensors in which the promoterless lux and gfp genes are fused under control of inducible promoters producing bioluminescence or fluorescence.
The location of the bone tunnels is a crucial factor for the success of the reconstruction surgery and the bone tunnels should be placed at the original insertion site of the ruptured ACL (van Eck et al. 2010; Chechik et al. 2013).
After mating, genomic DNA was harvested from individual males and PCR amplified with primers flanking the original insertion site to examine the potential P-excision site.
The UAS-Aβ42 flies used in the lifespan analysis carry additional copies of the transgene, generated by re-mobilization of the original insertion.
Similar(16)
After the muscle was separated from the globe, two needles were passed through each end of the original scleral insertion, with the needles pointing toward the center of the insertion site.
However, the segregation ratios of new insertions were affected only when the piggyBac element reinserted near the original donor insertion (representing a local hop).
This later can be a challenging problem since more or less extensive edits may have been performed on the original copy: insertion or removal of useless chunks of code, rewriting of expressions, transposition of code, inlining and outlining of functions, etc.
The integration site was identical in these clones indicating that they arose from the same original PB insertion.
Using a similar strategy, virulence was also restored to the original transposon insertion mutant (Kim53-K9), confirming that its attenuation resulted from elimination of nlpD expression and not from disruption of pcm expression (Fig. 5C, Table 2; note also that the Kim53Δpcm is as virulent as the wild type strain).
Related(20)
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underlying insertion
added insertion
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