Sentence examples for copies insertion from inspiring English sources

Biolistic transformations frequently lead to multi-insertion copies insertion in transgenic plants, and possibly cause higher expression levels of sgRNA and Cas9 protein.

Using the full build service platform at Knudra Transgenics, the plasmids pNU164 and pNU191 were constructed by inserting daf-16 cargo into MosI-mediated single copy insertion (MosSCI) vector backbones.

DNA blot analysis showed single copy insertion of transgene in SH41.

GSC, germline stem cells; IGF-1, insulin-like growth factor; IP, immunoprecipitation; mosSCI, Mos1-mediated single-copy insertion; MS, mass spectrometry.

The respective selection marker cassettes were used to derive mini-Tn7 elements that can be used for single-copy insertion of genes into bacterial chromosomes.

To detect the endogenously produced protein encoded by c30f12.4 and the location of C30F12.4 in worms, we constructed a 3×FLAG-GFP C30F12.4 transgene worm using the Mos1-mediated single-copy insertion (mosSCI) system.

Southern blot hybridization and inverse PCR analyses of genomic DNA demonstrated a precise piggyBac-mediated, single copy insertion of the pBac[3xP3-EGFP afm,Vg-DefA] transposon in each transgenic line.

This later can be a challenging problem since more or less extensive edits may have been performed on the original copy: insertion or removal of useless chunks of code, rewriting of expressions, transposition of code, inlining and outlining of functions, etc.

The multi-copy insertion of transcriptional reporter constructs into single genomic sites in mammalian cells provides the signal intensity needed to investigate transcriptional dynamics at a defined site.

The tagged gene in one pathogenicity mutant, with a single-copy insertion and greatly delayed formation of melanized microsclerotia on PDA agar medium, is a glutamic acid-rich protein 1 (VdGARP1); it shared no significant similarity to any known annotated gene.

Line #22 carries a single copy insertion on Chr5.