Sentence examples for existing insertion from inspiring English sources
There can be multiple primary insertions into the base element, as a new independent insertion event can occur adjacent to the existing insertion.
For examples with a predicted filled site > 1kb in length, or shorter insertions where a filled site was not detected using the standard assay, additional retrotransposon specific primers were designed and paired with the existing insertion site primers.
Currently, 2854 existing insertions are located within the coding introns of 1862 distinct genes (Nagarkar-Jaiswal et al., 2015), and MiMIC-like elements can now be placed in any gene of interest by CRISPR (Zhang et al., 2014).
Using a Δ2-3 transourcee source, we remobilized existing insertions of this P element and, via three different cross schemes, screened for new insertions onto the X-chromosome balancer FM7h (Heitzler 1997), the second chromosome balancer CyO, and the third chromosome balancer TM3 (see supporting information, File S1 for details of remobilization).
Nonetheless, some CIS pairs do break from this trend; for instance, insertions mutating Notch1 in the presence of an existing Rai17 insertion (p value = 0.001) appear to be more strongly selected for than insertions mutating Rai17 in the presence of an existing Notch1 insertion (0.0356).
The proposed cryogenic cooling loop represents an alternative solution between the usual water cooling method and the very efficient but expensive cryogenic system already existing for insertion devices.
We took advantage of an existing transposon insertion into the peel-1 gene, which is predicted to eliminate the function of peel-1.
Following the double restriction digestion, the genomic DNA fragment was cloned into a binary vector, pZH2B-GUS (Kuroda et al. 2010), with a pre-existing insertion of the beta-glucoronidase (GUS) gene.
Re-introducing the Ac transposase will lead to excision of existing DsDELGT4 insertions.
We showed that the Ds collection can be used to create additional enhancer and protein trap events by remobilization of the existing Ds insertions.
Summary: This Research Advance describes the creation of a genetic toolkit that allows for the (Recombination Mediated Cassette Exchange) RMCE of an integrated exon encoding for GFP with existing MIMIC insertions.
