Because the loop's inherent M/F is high enough, no activation bends or bends in the formed loop need be added before insertion.
Water was added via insertion of continuously flowing miniature water fountains through the base of the stainless steel chamber (Text S1; Figure S2).
On the C-terminus of NiG a triple Strep-tag was added by insertion of a DNA oligomer encoding the amino acid sequence (including vector-derived linker sequence) EEALSLEGPGAAGASGGG WSHPQFEKGSGGG WSHPQFEKGL WSHPQFEK-Stop at the XhoI and ApaI sites (individual Strep-tags underlined).
If this insertion is sufficient for pyruvate recognition, then adding the insertion to a modern apicomplexan MDH should convert the enzyme to an LDH.
Those include placing ads in content consumed on mobile devices and "dynamic ad insertion," which would allow targeted advertisements to be added to programming.
In this experiment, the synthesized PQDs, monoclonal antibody, and PQD-antibody conjugation were added to specimen insertion ports, named lanes 1, 2, and 3, respectively.
To obtain the proper plasmid constructs more efficiently, a negative bacterial selection marker, the ccdB gene, was added at the insertion site of oligonucleotides (Supplemental Figure 7A&B).
For cutting 50-μm vibratome sections, the stained (E13.5 and E15.5) specimens were inserted into 1 ml of 0.5%gelatine/30%BSA/20%/20 % sucrose/PBS, wherein 140 μl of 25%% glutaraldehyde was added immediately before insertion and incubated for 10 min.
For example, it is unclear without comparing feature coordinates at changed regions, whether a reported analysis based on a genome sequence downloaded in 'February 2004' would have considered the large 220-bp insertion added by SGD on 18 February 2004.
The total insertion force f needle was defined by adding the needle tip force f tip to the friction force f friction of the needle surface [4].
