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(G) Alignments of mutant sequences from embryos with SaKKH-BE3-mediated editing at the FANCF locus.
(D) Alignments of mutant sequences from embryos with BE3-mediated editing at the HBB locus.
(J) Alignments of mutant sequences from embryos with SaKKH-BE3-mediated editing at the DNMT3B locus.
Mutant sequences from synthetic TEM gene variants and from TEM gene variants in bacterial strains generated large increases in fluorescent signal relative to that from the reference sequence for TEM-1.
We identified 1652 mutant sequences from 1006 independent mutations that underwent clonal expansion.
Population compositions were determined by amplification of sequences across modified regions and cleavage with restriction enzymes that differentiated WT mutant sequences from each other.
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We had to omit this step because the wild type sequences in the databases are identical to the mutant sequences, apart from the single missense mutation.
The mutant sequences are numbered from 1 to 12. HAuCl4, sodium tris-citrate, NaOH and HCl were purchased from Xilong Chemical (Guangdong, China).
Structural distance between wild-type and mutant sequences was calculated from base pairing probability matrices (Sabarinathan et al. 2013).
The structural distance (d max ) between wild-type and mutant sequences was calculated from base pairing probability matrices [ 33].
The observed clouds also showed that there existed a fluctuating dynamic in these populations, as the shape of the clouds -- and gross amount of mutant sequences -- changed from one burst to another.
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