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The fact that ticks acquired the dbpAB mutant organisms from infected animals must also be considered.
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The proportion of genotypically mutant organisms that show the mutant phenotype.
In contrast, mutant organisms grew diffusely in liquid medium and produced a moderate degree of turbidity.
Despite the fact that mutant organisms could be detected in wild-type cultures by the mass-plating procedure, all our attempts to isolate mutants from cellulose-producing cultures by the indirect-select ion method of Lederberg and Lederberg2 have been unsuccessful.
RECENT studies1 with a cellulose-producing culture of Acetobacter acetigenum NCIB 8132 revealed that this culture contained mutant organisms which were unable to produce extracellular cellulose (celluloseless mutants).
The idea is to randomly introduce mutations into the DNA of thousands of organisms, screen for mutant organisms with a certain characteristic (such as failure to develop properly), and then map the genes affected in those mutants.
However because no INH tolerant mutant bacteria were recovered from untreated cultures, we cannot conclude on whether AA is more effective at producing INH-tolerant phenotype in WT bacteria than mutant organisms.
However, mutant organisms were detectable for at least ten days post-infection (Fig. 2).
On the basis of pathological data from patients with TSC, crucial aspects of any relevant model of TSC include elevated TOR (mTOR) activity and increased cell size within mutant organisms.
Since then, subsequent studies have revealed similar insoluble structures in a wide range of organisms, from cattle and sheep to humans [ 5] and cultured neuroblastoma cells expressing wild-type or mutant human PrP [ 6].
As proof of concept, we used this plasmid to express 12 different adhE genes (both wild type and mutant) from several organisms.
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