Sentence examples for mutant animals from from inspiring English sources

Consistent with this observation, we did not detect any Rho1 protein by western blot or by indirect immunofluorescence of fixed embryos in Rho1E(br)246 mutant animals from 12 hr after egg laying (the earliest time point we could unambiguously identify mutant embryos; Figure 2C and data not shown).

*, dcr-1 mutants are sterile, data shown are homozygous mutant animals from heterozygous mothers.

PCR templates were generated by cloning mutant animals from among the self-progeny of EG1000/CB4856 F1 hermaphrodites (described above) onto individual seeded plates.

In each ced-3 lf) ced-3 lf3(lf);pdr-1 mutand animals from our experiment, some undead ced-3 lfre ced-3 lfd as referenced also by the detailed protocol described in Schwartz.

Genomic DNA was isolated from mouse tail clips and genotyping to distinguish homozygous mutant animals from heterozygous and wild-type littermates was then performed either by PCR or by Southern blotting.

We then shifted lin-12(n676n930) mutant animals from the permissive temperature to the restrictive temperature at different larval stages and examined the vulval muscle morphogenesis and vm2 muscle arm development in the resulting adults.

However, in order to exclude that constitutive deletion of Rip3 in Casp-8LPC-KO/RIP3−/− mice resulted in general signaling defects of immune cells as a reason for the rescue of these double-mutant animals from hepatic fibrosis, we isolated and cultured monocytes from murine bone marrow of WT, Casp-8LPC-KO, Casp-8LPC-KO/RIP3−/−, and RIP3−/− mice.

With this technique we were able to collect datasets covering the whole organ of Corti to select snapshots of corresponding areas at defined orientation from control and mutant animals for comparison.

Specifically, our biochemical data indicate that SDHB is critical in preventing electron leakage from complex II, so that mutant animals suffer from increased oxidative stress and, as a result, are highly sensitive to oxygen and die rapidly [ 32].

Indirect flight muscles were dissected from 40-day-old control or ND2 mutant animals, or from 7-day-old parkin-null animals in phosphate buffered saline (PBS).

Additionally, we prepared RNA from wt animals as well as from early (F3, F7) and late (F11) generation trt-1 mutant animals, generated from backcrossed heterozygous trt-1 mutants.