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Ppt1 mutant embryos from Df(1 446-20, Ppt1A179T, and Ppt1S77F, and trans-heterozygous Ppt1 mutant crosses show a variety of phenotypes.
Prall et al. (2007) [30] used cDNA microarray analysis to compare the transcripts of mouse Nkx2.5 heterozygous and null mutant embryos from E8.0 to E9.5 and found that tbx5 is upregulated two-fold greater in null embryos at E8.0-E8.5 E8.0-E8.5
All analyses were confirmed using 2 5 biological replicates (e.g. mutant embryos from different litters of same stage).
A marked reduction in EphrinA7 expression prevents mesenchymal cells in the autopod of homozygous Hoxa13 mutant embryos from forming chondrogenic condensations in vivo and in vitro [ 57].
In par-2 mutant embryos from metaphase to telophase, GPR-1/2 shows a symmetric distribution and lower overall levels compared to wild-type.
Six hundred and ninety-six mutant embryos from tb233c/WIK incrosses were used for fine mapping with SSLP and SNP markers.
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RNAs were extracted from mutant embryos obtained from white seeds collected in developing siliques at 2 and 11 days after pollination.
In Ebf1 −/− mutant embryos, axons from the dLGN are misrouted inside the basal ganglia towards the amygdala.
Cofilin-1 mutant embryos, obtained from the Zebrafish International Research Center ZIRCC), were the result of mating wildtype AB female zebrafish with sperm from an AB zebrafish heterozygous for the hi3736aTg proviral insertion in the cfl1 gene [20].
Overall, our data suggest that defects in Axd mutant embryos result from over-expression of Grhl2.
Sce KO and Sce 33M2 mutant embryos derived from Sce KO and Sce 33M2 mutant germ cells, respectively, show comparably severe homeotic transformations (supplementary material Fig. S2).
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