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Eventually, we tested candidate genes in these regions with available lethal mutant lines from Bloomington.
In order to study the functional relationship between MIRO GTPases in Arabidopsis, we obtained independent mutant lines from publicly available seed collections.
Ten lines of 43 candidate mutant lines from the primary screen were confirmed to result in rapid GSC loss after clone induction in the secondary screen.
The mutational density may change after additional screening of other genes and/or more mutant lines from the population or changing the pooling strategy.
In stark contrast, mutant lines from all three geographic populations showed a significant decline in fecundity of up to 47 percent after between two and four generations.
We obtained 5041 insertional mutant lines from our mutant sources (Kuromori et al. 2006) and from the Arabidopsis Biological Resource Center (Columbus, OH).
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The identified mutant träge was outcrossed over five generations to clean the mutant line from background mutations before phenotypic analysis.
To validate the significance of the mutation in the GmGIa gene, we screened a mutant line from the mutagen-treated libraries.
Genomic DNA was extracted from mutant lines selected from the flanking sequence tag (FST) database.
Two mutant lines derived from 'Bay' (KK24, MM106) were crossed to the public soybean cultivar 'Williams 82'.
Previous studies have focused on examining the functional effect of single genes on social interactions in D. discoideum, using mutant lines obtained from screening in laboratory strains [ 20, 34, 35].
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