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10.7554/eLife.05061.009 Figure 4. Kif5aa mutant larvae show no activity in RGCs and no synaptic transmission to tectal cells.

Zfrp8 mutant larvae show a disruption of homeostasis in the lymph gland and vast lymph gland overgrowth.

Another complication is that DLG mutant larvae show dramatic underdevelopment of the subsynaptic reticulum (SSR), a dense infolding of postsynaptic membrane that appears during larval NMJ growth [ 16, 17, 19, 24].

This is of particular interest since homozygous 12-day old SmD3 mutant larvae show overgrowth of the brain, hematopoietic organs and imaginal discs and die during third instar larval or pupal stages [ 51].

Consistent with the results reported by Pickart et al., apoc2 mutant larvae show a slightly delayed yolk consumption (Fig. 3A-G) and the mutant larvae are smaller than WT larvae.

A precisely timed quantification of larval growth indicates that although dGPHR mutant and wild-type larvae grow equally until late mid L3 stage (96 hr AED), dGPHR mutant larvae show growth deceleration at late L3 (between 96 hr and 120 hr AED) (Fig. 2D).

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Consistent with this finding, Rheb hypomorphic mutant larvae showed a significantly reduced number of boutons per unit muscle area compared to heterozygous controls (Fig 2C, D).

Evaluation of mRNA levels by RT-PCR in homozygous mutant larvae shows that the sumo04493 mutation leads to an approximately 5-fold decrease in the level of sumo transcripts (Figure S3).

If stained with an anti-DAMB antibody [40], DAMB mutant larvae showed a strongly reduced expression in the larval brain (Figure 4I) compared to the rosy control (Figure 4J).

Careful examination of the wnt2O mutant larvae showed no visible defects in either the patterning or size of the muscles (Fig. S1: WT = 26180±1987 µm2, n = 6, wnt2O = 27130±1473 µm2, n = 8, p = 0.70).

At 1 hour after heat shock, both wild-type and yata mutant larvae showed induction of APPL-FLAG in the ventral ganglion, axons and the cell bodies of muscles (Figure 6C, D, I, J, O, P), whereas APPL-FLAG was not localized in the neuromuscular synapses (Figure 6O, P).

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