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These mutant larvae displayed a delay in pupation, likely caused by the continuous growth of the disc proximal regions.
All otic sensory patches in the mutant larvae displayed reduced numbers of hair bundles, as well as defects in stereocilia organization at 5 dpf (Fig. 3G I).
Unlike wild-type and in striking contrast to nutrient starvation, rapamycin-fed buffy mutant animals did not initiate autophagy faster than wild-type, but were instead slower: fat bodies from buffy mutant larvae displayed 'zero to minor' LTR staining even after 80 minutes of rapamycin feeding.
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The shopper mutant larvae display the same but stronger behavioral deficits than those noted upon ensheathing glial cell-specific silencing of shopper (Fig. 4e g).
We previously reported that dC1GalT1 synthesizes T antigen expressed in hemocytes, lymph glands, and the central nervous system (CNS) and that dC1GalT1 mutant larvae display decreased numbers of circulating hemocytes and excessive differentiation of hematopoietic stem cells in lymph glands.
In fact, DLG mutant larvae display larger excitatory postsynaptic potential amplitudes [ 17].
Similar to the effect on CG6770 and cabut, TOR-mutant larvae displayed increased d4E-BP transcription, which was completely suppressed by the fkh1 or the fkh6 mutation (Figure 7B).
Heterozygous T70I mutant sod1 larvae displayed the largest variability in r-value (overlap coefficient, where 1 is complete overlap and 0 is no overlap), and there was no significant difference between the r-values of WT and heterozygous larvae.
Although Tubulin levels were similar in wild-type and mst mutant brains, mst larvae displayed substantially reduced levels of α- and β-Tubulin compared to wild-type larvae; this difference likely reflects variable susceptibilities of different tissues to loss of components of the Tubulin-folding pathway, as previously reported for Drosophila Mgr [ 17].
Our studies demonstrate that as early as at 3 dpf, apoc2 mutant larvae already display remarkable hyperlipidemia, and in 6 dpf embryos, dramatic differences in circulating neutral lipids were detected with ORO staining and confirmed with biochemical measurements of TG and TC levels in whole-body homogenates (Fig. 4).
Homozygous mutants displayed extra neuromasts, whereas wild-type and heterozygous larvae displayed only seven to nine neuromasts on one side of their trunk.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com