Here, both control and Fgfr1 mutant samples show that palatal shelves are blocked by the tongue.
Accordingly, two melting peaks are observed in the first-derivative plot of normal samples, while mutant samples show another peak, generated by heteroduplexes melting, the height of which is proportional to the fraction of cells bearing the mutation.
Repetitive sample measurements with representative wild-type, heterozygote, and mutant samples showed that greater than 9 SDs separated the means of heterozygote and homozygote sample populations.
Mutant samples showed an abnormal profile across the entire range of template amounts tested and could be readily identified (not shown).
Samples 33K and 75K were confirmed as wild type, while mutant samples showed the following variation frequencies: 22.8% G12V for 51K, 35.7% G12C for 97K, and 38.9% G12D for 81K.
(F ) Quantitative analysis of Ret immunoreactivity in C4da somata of wildtype and Ret mutant samples showing the signal over background (ΔF/F, mean ± SD, n = 5, p < 0.001, Student's two-tailed t -test).
(B ) Quantitative analysis of phospho-Ret immunoreactivity in C4da somata of wildtype and Ret mutant samples showing signal over background (ΔF/F, mean ± SD, n = 3, p < 0.05, Student's two-tailed t -test).
If the mutants showed the same wetting as the wild types, the probability of an individual mutant sample showing Wenzel wetting would be 0.645 and the probability of 14 mutant samples (out of 14) showing Wenzel wetting can therefore be calculated as 0.002 (exact value from binomial test).
A Gene Set Enrichment Analysis demonstrated that genes characteristic for hematopoietic progenitors were expressed at higher levels in samples harbouring wild-type N-RAS while genes associated with mature blood cells rather than progenitor and stem cell compartments were strongly enriched (up-regulated) in the N-RAS mutant samples (not shown) [35], [36].
Wild-type and mutant cartilage protein samples showed comparable levels of COMP when normalized to Ponceau staining and quantified by densitometry (data not shown).
Epidermal growth factor receptor copy number and EGFR mutation may be related, as out of five EGFR mutant samples, two samples showed high polysomy and one sample showed EGFR amplification.
