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The difference will be the number shared by two DNA sources or multiple sequencing platforms.
The genome and transcriptomes of A. indica were sequenced using multiple sequencing platforms and libraries.
Multiple isolates from different regions of the tree and sequence data from multiple sequencing platforms were analyzed.
It should also be pointed out that not all assemblers are designed to work with data from multiple sequencing platforms.
Therefore, integration of multiple sequencing platforms is one strategy for de novo sequencing when there is no reference genome available [ 32].
Thus there is a need for a realistic, cross-platform NGS simulator, as multiple sequencing platforms are likely to persist, each with their own strengths and weaknesses [ 14].
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Nevertheless, the data presented here provide useful information that will aid in gauging the performance of different capture approaches and assessing how generalizable enrichment method performance will be across multiple sequence platforms.
76 The multiple commercial sequencing platforms developed (Table 2) are able to sequence millions of DNA fragments in parallel, and the different sequencing chemistries result in differences in total sequence capacity, sequence read length, sequence run time, and the final quality and accuracy of the data.
Therefore, it is necessary to develop multiple sequencing approaches and platforms, including the third-generation long read technologies, high-quality long-insert clones and new assembly algorithms.
Nevertheless, few genomes have been subjected to both WGS and ES, using multiple next generation sequencing platforms.
Multiple next-generation sequencing platforms are available (Table 2), but the two most commonly used methods in metagenomics studies are Illumina sequencing-by-synthesis and Roche/454 pyrosequencing.
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