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However, in some samples this interpretation is difficult, i.e. when (almost) equally quantitative LH variants are present or when multiple sequencing primers result in the presentation of different dominant types.
This technology is based on the use of multiple sequencing primers in the same pyrosequencing reaction.
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To our knowledge, the distance from sequencing primers to multiple cloning site (MCS) is not too long and 100 nucleotides were used as threshold.
Sequences obtained from the same regions of multiple strains on resequencing arrays were used to design gene-specific sequencing primers for the genomic fragments.
Also, the generation of suitable insert-specific sequencing primers can require a huge effort when large numbers of clones have to be sequenced, each with multiple primers.
Sequences for bisulphite sequencing primers.
PCR and sequencing primers.
Sequencing primers for pDECKO.
We used multiple overlapping PCR and Sanger sequencing primer pairs (electronic supplementary material, table S2) and aligned the resulting genomes using CodonCode (http://www.codoncode.com. CodonCode Corporation).
To discover specific primers, a multiple sequence alignment (MSA) of all GenBank FeLV env sequences (available as of June 2006) was compared with the MSA of endogenous FeLV (enFeLV) and FeLV-C env entries.
The 'core' sequences were used to create multiple sequence alignment and design the primers.
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