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Exact(17)
The scope of the project required multiple sequencing runs.
All gaps between contigs could be closed by combining the results from multiple sequencing runs.
However, unlike identification, characterization of pathogens is likely to require higher titers, multiple libraries and/or multiple sequencing runs.
Data analyses and comparison of variants between the multiple sequencing runs were performed using custom Perl scripts.
The eight lanes of sequences were generated over multiple sequencing runs that were run to variable read lengths.
Where multiple sequencing runs were generated on the same library, BAM files were combined into a single BAM file with Picard MergeSamFiles prior to identifying and removing duplicates.
Similar(43)
Three MBC samples were also run at least two times across multiple sequence runs to examine for run-specific variation.
Final coverage of the genome was 4-fold with the majority sequenced on both of the strands or, where bidirectional sequencing was impractical, with multiple sequence runs on the same strand.
We constructed multiple sequence alignments, ran HHpred searches [ 21] and analyzed gene context for all members of arCOGs that contain identifiable (although in some cases, apparently inactivated) DHH domains.
The protocol's throughput is also enhanced by its highly parallel nature, which we leveraged to co-load libraries for multiple RNAs on single sequencing runs ('Materials and methods').
Briefly, multiple sequence alignments were run using the MAFFT (online version) and the output FASTA format data were plugged into Mega software.
More suggestions(15)
multiple sequencing providers
multiple sequencing profiles
multiple sequencing studies
multiple simulation runs
multiple sequencing lanes
multiple sequencing data
multiple sequencing platforms
multiple sequencing methods
multiple sequencing strategies
multiple sequencing alignments
multiple sequencing approaches
multiple sequencing technologies
multiple sequencing methodologies
multiple sequencing errors
multiple sequencing primers
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