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Exact(7)
The overall effectiveness of our approach as a tool for the routine management of GM mouse lines, was demonstrated by cryopreserving and recovering 735 GM lines maintained on twelve genetic backgrounds, including 527 GM lines maintained on the C57BL/6J background.
In 10-month-old mice, no overt sprouting in any of the mouse lines was observed.
Production of Scgb3a2-transgenic mouse lines was confirmed by Southern blotting of genomic DNAs isolated from clipped mouse-tails.
Fold difference of transgenic mouse lines was compared to wild type CD1 mice and transgene copy number was estimated based on this fold difference.
The level of endogenous 5-HT2C receptor mRNA expression in both C2CR mouse lines was unaltered compared with the controls (data not shown).
Genotyping for all other mouse lines was carried out as reported (Buscher et al., 1998; Lu et al., 2002; Schedl et al., 1996 ).
Similar(52)
After confirming the functionality and specificity of the construct in vitro, transgenic mouse lines were generated by pronuclear DNA microinjection.
Both mouse lines were maintained on the B6/CBA crossed background.
These mouse lines were interbred to generate Ndst1f/fMMTVCre+ (mutant) and Ndst1f/fMMTVCre− (wildtype) mice.
Homozygous females of both mouse lines are unable to produce offspring.
However, the reason for this discrepancy in these two Oligl−/− mouse lines is still unclear.
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