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Moreover, both transgenic mouse lines showed browning of WAT with elevated Ucp1 and Cidea expression (Figure 5I), comparable to previous reports6,7,8, without BAT activation (Supplementary information, Figure S9B).
Compared with the wild-type mouse, they found that 15 mutant mouse lines showed significantly decreased pulmonary metastatic foci and 8 mutant mouse lines increased.
During conditioning both mouse lines showed increased fear responses as assessed by freezing behaviour.
Interestingly, 3/5 mouse lines showed expression in the newborn (fig. 5A, D, G) and adult brain (fig. 5B, C, E, F, H, I).
Another preferential site of expression was the developing CNS: 3/5 mouse lines showed transient fiZ expression in the spinal cord and hindbrain at E11.5 to E12.5 (fig. 4C D).
Again, all three mouse lines showed a significant variation over the day regarding the time spent exploring the cage (Friedman-tests: N = 12 for each line, Chir2 = 135.5 149.5, df = 23, all p<0.001; see Fig. 3).
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There are few naturally occurring or engineered mouse lines showing as severe a skeletal myopathy as observed with ectopic expression of CAR in the homozygotes.
The precise analysis of BAC transgenic mouse lines shows that 100% of the MSNs express D1R or D2R or in a minority of cases both.
All mouse lines show abnormal tissue formation caused by the disrupted cell cell junctions.
The phenotypes observed in Megf8 L1775P and Megf8 − mouse lines show a striking resemblance to those observed in mice lacking BMP4 signaling (Table 1).
It is noteworthy that all three mouse lines show similar pathologies in mesenchymal tissues, with the skeletal system, being predominantly affected.
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