Mouse lines were maintained in a mixed background.
All mouse lines were established on a C57Bl/6 background.
After confirming the functionality and specificity of the construct in vitro, transgenic mouse lines were generated by pronuclear DNA microinjection.
Using BW 5147 T cell hybridomas isolated by fusion with spleen and lymph node cells from NOD female mice, two T cell receptor transgenic NOD mouse lines were produced.
These mouse lines were interbred to generate Ndst1f/fMMTVCre+ (mutant) and Ndst1f/fMMTVCre− (wildtype) mice.
Both mouse lines were maintained on the B6/CBA crossed background.
The single Msh2−/−, Msh6−/− and Msh3−/− mouse lines were generated and reported previously [10], [27], [49].
All mouse lines were maintained under specific pathogen-free conditions at the POSTECH animal facility under institutional guidelines.
However, in many published papers, investigators have not specifically mentioned which of these different MMTV-Cre mouse lines were used in their studies.
Thymocytes and spleen B cells, isolated using the MACS separation technology (Miltenyi Biotech, Auburn, CA) from BAC transgenic mouse lines were analyzed by real-time, reverse transcriptase PCR.
Thymocytes and B cells of analyzed transgenic mouse lines were fixed in 10 ml RPMI medium with 1% formaldehyde at room temperature for 10 minutes.
