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(Details on MART® and CART analyses are reported in Text S1, and Figure S1) Of the prognostic thresholds identified, the most robust phenotypes are A1 and A2, and B1 and B2 (Table 2).
Similarly, the next most robust phenotypes are defined by the number of embryos and cleavage arrest rate, such that for cases with ≥6 embryos, those with cleavage arrest rate >14.6% are 3.0 times more likely to result in no pregnancy than those with cleavage arrest rate ≤14.6% 955% CI, 1.9 to 4.9).
The 33 most robust phenotypes are all inviable.
Because the rotarod deficits in the HD-N171-82Q line 81 mice are relatively modest, we chose to focus on the most robust phenotypes, which include early death, failure to gain weight, brain atrophy and inclusion pathology.
To elucidate the relative contributions of Dw1 and Dw2 to dwarfing of the scion, we examined three of the most robust phenotypes associated with dwarfing, i.e., early flowering (spring of year 2), final TCA (year 7) and overall visual assessment (year 7) of scions grafted to rootstocks carrying various combinations of Dw1 and Dw2.
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For that purpose, we used the 1∶5 line substrate throughout this study since it leads to the most robust phenotype in terms of neurite length.
However, within the scope of the paper, we focused on two peptidergic systems whose manipulation yielded the most robust phenotype.
The wild type phenotype (in both glucose and galactose growth media) is among the most robust viable phenotypes as expected, since the high number of hits during optimization is strongly dependent on the model's ability to capture wild type viability.
First, we looked at the most robust neural phenotype that has been linked to VEGF-NRP1 function.
Of the 9 other xylose-positive yeasts that we identified, the strain with the most robust xylose phenotype was CBS1502, which showed a reproducible increase in both optical density and cell number relative to a xylose-negative, S. uvarum control strain.
The most robust breathing phenotype in RTT mouse models that has been reproducibly observed by different laboratories and in different mouse strains is abnormal variation in respiratory cycle length in room air, including respiratory pauses and periods of tachypnea associated with decreased expiratory time and increased mean breathing frequency (Table 2; supplementary material Table S4).
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