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Exact(5)
All chimeras whose most likely transcript was either NM or XM were deemed to be a small RNA-mRNA chimera.
For the chimeric reads, the most likely transcript was deemed to be the transcript which was most abundant in the data.
Since ASPic is also able to detect the most likely transcript variants for a gene, we propose here two simple extensions to ASPic-GeneID that allow the prediction of alternative splicing transcripts.
In order to estimate the most likely transcript abundance distribution for the single cells analysed here, we varied the scale and shape parameters of the distribution to better fit the experimental distribution observed in the 'half-vs-half' microarray data.
To identify the most likely transcript, every read of the CLASH data was searched against the human Ref-Seq database using blastn, word size 7, default scoring matrix, 20 maximum hits.
Similar(55)
Most likely, transcripts of this size were synthesized in vivo given the RT-PCR results obtained and the locations of the primers used on the gene sequence.
These results convincingly show that a substantial proportion of nTARs are most likely transcripts of protein-encoding genes rather than pseudogenes or regulatory RNAs.
Although more transcriptome sequences could be produced from de novo assembly using dataset 3 than dataset 1, the mapping ratio could not be improved (Table 2), indicating that the additional transcripts from dataset 3 are most likely transcripts that are expressed in tissues other than the leaves of tea plants.
It is noteworthy that current genome annotation predictions estimate around 12000-17000 gene models, so most likely some transcript models are still missing on the platform, however an updated version of the array can easily be established in the future.
Notably, it does not provide any location-specific information detailing where these over-represented codons fall in the gene, and there will most likely be transcript regions that are more severely biased in certain codons and thus represent key regulatory motifs.
To that point, it is not possible to exclude other hypotheses such as nonsense mediated decay (NMD) in which most likely aberrant transcripts with a PTC would be largely degraded.
Related(20)
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