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Autumn mothers and dwarf eldest daughters had the most similar transcript expression profiles (77% bootstrap support; Figure 3).
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The remaining proteins were associated to the most similar transcripts, provided that there were up to five non-contiguous gaps or substitutions (28 proteins did not satisfy this condition).
Given these facts we wondered whether ABA and/or ethylene and/or cytokinin signaling are involved in desiccation tolerance in K. crenulatum, and searched for the most similar transcripts to the K. flaccidum proteins reported to be putative orthologues of these 3 (ABA, cytokinin, ethylene) plant phytohormones signaling components.
Autumn regular daughters had unique gene expression profiles, but were most similar in transcript expression to other females assayed during the autumn, non-reproductive phase of the colony cycle.
It was our hypothesis that the post blood meal midgut transcript abundance is most similar to the sugar fed midgut transcript abundance prior to a blood meal.
It was identified as a transcript most similar to a hypothetical protein, TTHERM_02641280, from T. thermophila.
Although most genes showed a similar transcript level in the tpk1 tpk2 tpk3 msn2 msn4 and tpk1 tpk2 tpk3 yak1 strains, some notable exceptions were observed (Table 3).
The transcript most similar to the characterised GalUR from strawberry (XM_002285191.1) was detected at very low levels in young berries, and not at all in the other samples.
Whilst those GSTs most similar to the rotifer transcripts all show expression responses to electrophilic stress [ 38], interestingly GST-5 occurred in an expression cluster of strongly regulated dauer genes (WBPaper00024393; [ 39]).
Hemocyte and gill transcriptomes shared 60%% of the transcripts while mantle and muscle transcriptomes were most similar, with 77 % shared transcripts.
Nonetheless, of the four mutants, nin plants had a transcript profile most similar to wild-type.
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