Ribosomal protein (RP) (GenBank: FG618944) was the most abundant transcript expressed during desiccation, suggesting the possible involvement of transcriptional regulation of ribosomal proteins during desiccation stress.
The most abundant transcript in FDE list was SDR65C, reflecting its importance in laticifer differentiation.
Other than three unnamed protein products, a ncRNA was one the most abundant transcript at all stages, peaking at the green stage and decreasing at the last two stages.
β-lactoglobulin, a major nutrient protein29, was the most abundant transcript and peptide in the early lactation sample and was the second most abundant peptide in the late lactation sample.
TGF-β represented the most abundant transcript while IL-4, IL-5, IL-6, IL-10, IL-12, IL-18 and TNF-α were present at levels approximately 1000-fold less.
For seven genes Cq values ranged between 23 and 25 cycles, and only one gene – GAPDH – have the most abundant transcript level (mean Cq = 18,6).
In all cells where it was measured, para was the most abundant transcript, and in 84% of cells where it was measured, shaw was the least abundant.
The binomial distribution is often approximated by Poisson distribution Poisson with λ = π⋅N when N is large, π is small, and π⋅N is moderate, which is precisely the case even for the most abundant transcript.
The next most abundant transcript (BpA0299) contains a complete 1593 nt ORF with weak BLASTX matches (E>0.01) to insect chitinases and has three chitin binding Peritrophin-A domains identified by hmmpfam.
The most abundant transcript was an SSU rRNA not eliminated in the mRNA purification (EST BpA0300); other transcripts with more than 1% of the total ESTs are listed in Table 2.
This mRNA is considered monocistronic and it is the most abundant transcript in all transgenic lines.
