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Our results show that in Rio, down-regulated genes outnumber those that are up-regulated by a factor of 2. The most reduced transcript has a fasciclin domain.
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The transcripts for the IL-17 receptor, MHC-I heavy chain, IL-6 receptor alpha chain, insulin receptor, and IL-16 receptor were among the most strongly reduced transcripts.
Most OR genes show dramatically reduced transcript levels in Emx2 knockout mice [ 30].
Whereas most RMP-fam-a and RMP-fam-b members had reduced transcript levels in gametocytes compared to asexual blood stages, a large cluster of pir genes were up-regulated (at least a fold change of 2) in gametocytes.
The stem libraries showed reduced transcript diversity following X.
The results exposed a striking positional effect in all 32 yeast chromosomal arms: whereas most genes reduced their transcript levels by several fold, genes situated at <100 kb from the chromosomal ends gradually escaped from the transcription stall.
A dnaK-specific probe, revealed a 3.5 kb-transcript in Northern blot analysis, most probably covering the hrcA operon, and confirmed reduced transcription levels in BB1305 at 37°C (data not shown).
Among the transcripts most strongly reduced in AtGRP7-ox plants was AtGRP8 previously shown to be under negative control by AtGRP7 [ 20, 24], validating the strategy.
Gratifyingly, among the transcripts most strongly reduced in AtGRP7-ox plants is AtGRP8 previously shown to be under negative control by AtGRP7 [ 20], validating the strategy used to identify candidate targets.
Most conspicuously, strain 6399 (milk isolate) appeared to have a largely reduced rpoN transcript amount, which also seemed to negatively affect luxS transcript, and matched the low observed in vitro motility of this strain.
Seventy-four annotranscriptscripts were differentially expressed between pre-and post-training states and among the 58 genes with decreased expression, MSTN mRNA transcripts were the most significantly reduced (-4.2-fold, P = 0.0043) [ 29].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com