Sentence examples for monitor phosphorylation of from inspiring English sources

That inhibition was not observed strongly suggests that Met oxidation is not affecting our ability to monitor phosphorylation of Ser.

In order to monitor phosphorylation of maltose, conversion of ATP to ADP was enzymatically coupled to oxidation of NADH (via pyruvate kinase and lactate dehydrogenase), and the latter monitored fluorimetrically (excitation 340 nm and emission 450 nm).

To monitor phosphorylation of USP28, the above reaction was carried out using 10 µM substrate (peptides corresponding to amino acids 543 557 [TCLQRWRSEIEQDIQ] or 892 906 [YSLFRKVSVYLLTGL] in USP28) diluted in 1X kinase buffer.

The fluorescence signals emanating from the antibody complexes that were used to monitor phosphorylation of the three tyrosine residues cannot be compared because of the different affinities of the primary antibodies for the epitopes, and distinct fluorescence brightness and binding affinity of the labeled secondary antibodies.

To monitor phosphorylation of paxillin, cells were stimulated as described below, rinsed once with ice-cold PBS and lysed for 30 min at room temperature (21°C) with rocking in SDS sample buffer [2 mM EDTA, 2% SDS, 50 mM Tris/HCl (pH 6.8), 10% glycerol, 5% 2-mercaptoethanol and 0.01% Bromophenol Blue].

To investigate this, we monitored phosphorylation of the Nodal intracellular signal transducer Smad2 in oep mutants with a normal or down regulated expression of rasl11b.

JNK activation was determined by monitoring phosphorylation of JNK (Thr183 and Tyr185) and c-Jun (Ser63), which is a substrate of JNK.

Confluent BAEC were stimulated with 5 mM 2-DG for 5 to 120 min, and AMPK activation was measured by monitoring phosphorylation of AMPKα-Thr172.

Using both untransfected and transfected cells, we monitored phosphorylation of STAT1 and STAT2 under the same experimental conditions as in Figure 3B.

To determine if low pH enhances F− -mediated stress, we treated LS8 cells with F− at pH 6.6 or pH 7.4 and monitored phosphorylation of JNK and c-jun.

JNK activation was determined by monitoring phosphorylation of JNK1 and total expression of this protein.