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On the other hand, Molina et al. [28], in a study of phosphopeptides in human embryonic kidney cells, identified phosphorylation of, unequivocally, eEF1A1 at Tyr29 and Ser163.
Besides, we identified phosphorylation of 11 BdCDPKs and 4 BdCDKs, which may function in defense response and regulation of cell division and differentiation [ 57– 57].
Previous studies had identified phosphorylation of two tyrosine residues (Y653 and Y654) as the key step in the shift towards an activated form of FGFR1 (Furdui et al., 2006).
A global phosphoproteomic study of a melanoma tumour identified phosphorylation of LRRK2 at Ser as one of 5600 phosphorylation sites catalogued on 2250 proteins, but this was not investigated further [ 21].
Because anti-MST3(275–393) mAbs fail to recognize FLAG MST3 FL) following calyculin A exposure, the phosphorylation site(s) probably lies between residues 275 and 393 and is distinct from the previously identified phosphorylation of MST3 on Thr of its activation loop [ 19].
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These correspond to 3,994 uniquely identified phosphorylations of 2,214 peptide sequences of 1,252 proteins (Additional file 1: Table S1), including several conserved protein kinases, discussed below.
Finally, we identify phosphorylation of STAT3 as a novel pathway activated by PPARβ/δ and show that inhibition of STAT3 phosphorylation blocks disease development.
To reconfirm the presence of potential MPK phosphorylation sites in OsAux/LAX1, we conducted in-silico prediction to identify phosphorylation of the OsAux/LAX1 protein using the kinasephos2.0 server [ 39].
Our results has uncovered a previously unrealized role of GSK3βY216 phosphorylation: it triggers GSK3β recruitment of β-TrCP and subsequent GSK3β monoubiquitination by β-TrCP, an event required for Wnt-induced inhibition of β-catenin degradation, thus identifying phosphorylation of GSK3βY216 as an 'activator' of the Wnt/β-catenin pathway.
The availability of large numbers of experimentally identified phosphorylation sites, and of natural variation datasets in Arabidopsis thaliana prompted us to analyze the effect of non-synonymous SNPs (nsSNPs) onto phosphorylation sites.
The kinase prediction tool NetPhosK 1.0 [ 33] predicts the identified phosphorylation site Ser of Rh2b CPD to be a substrate of either CK2 or PKC (protein kinase C).
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