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The thermocycle protocol lasted for 30 s at 95 °C, followed by 40 cycles of 5-s denaturation at 95 °C, 34-s annealing/extension at 60 °C, and then a final melting curve analysis to monitor purity of the PCR product.
Reagent blanks helped to monitor purity of the reagent used.
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The protocols were 1 cycle at 95 °C for 3 min, followed by 40 cycles at 95 °C for 15 s, annealing at the optimum temperatures for 30 s, 72 °C for 30 s and melting curves data were analyzed to monitor the purity of the PCR product.
To monitor the purity of mitochondrial and cytosolic fractions, COXIV and GAPDH immunoreactivities were assessed as mitochondrial and cytosolic specific markers, respectively (Figure 4B).
To monitor the purity of synaptosomal fractionations, we used PSD-95 as a postsynaptic marker and synaptophysin as a presynaptic marker.
ICAM-1 expression was also used to monitor the purity of mouse MCs (Supplementary Fig S7).
The PIN was read by a power meter that also monitored the purity of the input signal; while the PREF value was obtained directly by means of a spectrum analyzer.
The authors did not monitor for the purity of these analyses by probing for an abundant inner membrane protein.
To explore the enantioselectivity of lipases toward this dynamic system, chiral chromatography, supported by Mosher analysis and XRD (see the Supporting Information), was employed to monitor the enantiomeric purities of the ester products selected by the enzyme.
The reactions were monitored by GC MS analysis and the purity of the desired products was evaluated by NMR spectroscopy.
The purity of Tregs was monitored via FACS.
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