Sentence examples for monitored phosphorylation of from inspiring English sources

Using both untransfected and transfected cells, we monitored phosphorylation of STAT1 and STAT2 under the same experimental conditions as in Figure 3B.

To investigate this, we monitored phosphorylation of the Nodal intracellular signal transducer Smad2 in oep mutants with a normal or down regulated expression of rasl11b.

To determine if low pH enhances F− -mediated stress, we treated LS8 cells with F− at pH 6.6 or pH 7.4 and monitored phosphorylation of JNK and c-jun.

We also monitored phosphorylation of the transcriptional repressor Mig1p, which is targeted by Snf1p under low glucose conditions.

Thus, in order to estimate the time course of PDK activation, we monitored phosphorylation of PDK itself at S241 in the activation loop.

We monitored phosphorylation of NCC employing different previously characterised phosphospecific antibodies recognising major SPAK NCC phosphorylation sites (Thr46, Thr50, Thr55, Thr60 and Ser91) (10).

JNK activation was determined by monitoring phosphorylation of JNK (Thr183 and Tyr185) and c-Jun (Ser63), which is a substrate of JNK.

Confluent BAEC were stimulated with 5 mM 2-DG for 5 to 120 min, and AMPK activation was measured by monitoring phosphorylation of AMPKα-Thr172.

JNK activation was determined by monitoring phosphorylation of JNK1 and total expression of this protein.

That inhibition was not observed strongly suggests that Met oxidation is not affecting our ability to monitor phosphorylation of Ser.

These observations were confirmed at protein level by monitoring phosphorylation of Stat3 and extracellular signal-related kinase (Erk) 1/2.