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A Cu standard (NIST 976) was added to the purified Zn fractions and a Cu (NIST 976) + Zn (JMC 3-0749 L) standard mixture was run as a brack standard, thus allowing to perform different corrections.
Three µl of the reaction mixture was run on 18% polyacrylamide/7 M urea 45 cm long gels (ratio of AA∶BAA = 37∶1).
The deproteinized reaction mixture was run on an HPLC using a Beckman reversed phase Ultrasphere ODS 5 µm C-18 column (4.6 mm ID, 150 mm length) with isocratic flow with 5 mM potassium phosphate (pH 7.0), 5 mM tetrabutylammonium dihydrogen phosphate, and 5% (v/v) acetonitrile, while monitoring elution at 260 nm.
The mixture was run through a Sephadex column to elute unbound isotope.
Finally, all of the reaction mixture was run on 8% optimized native PAGE.
PCR reaction mixture was run in Applied Biosystems Prism 7300 Sequence Detection System instrument utilizing universal thermal cycling parameters.
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Aliquots of the reaction mixture were run on a 6% denaturing acrylamide gel.
After amplification is finished, a 20 μl portion of the reaction mixture is run in a 2% agarose gel.
15 μl samples of this mixture were run on a 4 20% Mini-PROTEAN TGX gel (Bio-Rad, Hercules, CA, United States).
Negative controls using distilled water instead of cDNA in the PCR mixture were run in parallel to exclude genomic DNA contamination.
The reaction mixture were run on a 7%polyacrylamide/0.55 × TBE gel containing 2.5% glycerol at 100 V for 80 min and then transferred onto Hybond N + nylon membrane (Amersham, USA).
Related(20)
material was run
compound was run
solution was run
mixture was cultured
mixture was strained
mixture was monitored
mixture was set
mixture was poured
mixture was degassed
mixture was sampled
mixture was prepared
mixture was filtered
mixture was spread
mixture was vortexed
mixture were run
mixture was shook
mixture was heated
mixture was kept
mixture was separated
mixture was touted
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