Sentence examples for mixture was lysed from inspiring English sources

For IPs from proteoliposomes, 40 μl liposome mixture was lysed in 1 ml buffer containing 50 mM Tris-HCl, 150 mM NaCl, and 1% Triton X-100.

The mixture was lysed using FastPrep-24 (MP Biomedicals) at 6.5 m/s, 20 s/pulse × 3 pulses with 5-min intervals on ice.

The cell and protein mixture was lysed by brief sonication at 20 W using three 10 s continuous cycles (Sonicator 3000 from Misonix, Farmingdale, NY).

The mixture was lysed using a FastPrep-24 (MP Biomedicals) homogenizer with 3 pulses (6.0 m/s, 20 sec/pulse); pulses were separated by 5-min intervals during which the samples were kept on ice.

The cell mixture was lysed in a 15 μl volume of solution containing 0.3%% v/v Nonidet P-40 detergent (Sigma), 500 ng random hexamers (Pharmacia, Uppsala, Sweden), 20 U of RNasin (Promega, Madison, WI) and 10 mM DTT.

The microbial cell was lysed with sonication and SDS.

To release the expression product, the cell was lysed sonication.

Donor fraction was lysed in 75 μl of Buffer C (10 mm Tris HCl [pH 7.6], 100 mm NaCl, 10% [w/v] SDS plus protease inhibitor mixture).

After the fluorometric readings for resorufin were taken, the reaction mixture was aspirated and the cells were lysed with CelLytic-M (Sigma-Aldrich Co). to obtain protein measurements using a BCA assay kit (Pierce Biotechnology, Inc., Rockford, IL, USA).

The cells were lysed in a mixture of dimethyl sulfoxide (DMSO) and glycine (0.1 M), and the absorption was measured at 550 nm using a microplate reader (Bio-Rad, Nazareth, Belgium) (see Supplementary Materials and methods for details).

Cerebral cortex tissues were lysed using a mixture containing 50 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 120 mM NaCl, 0.5% NP-40, pH 7.5, a protease inhibitor (Roche Diagnostics) and 1 mM PMSF.