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Protein-encoding regions of each transcript were predicted using Transdecoder with a minimum protein length of 50 amino acids.
From this reference transcriptome, a total of 41,460 proteins were predicted by TransDecoder with minimum protein length of 50.
Note that although our method can detect shorter proteins than many other methods, we still have a detection threshold of minimum protein length.
To verify de novo assembly quality, putative open reading frames (ORFs) within transcript sequences was identified by TransDecoder (http://transdecoder.sourceforge.net/) at minimum protein length of 50.
ORF search was set at a minimum protein length of 48 nt, which is known from the leader peptide of ilvB transcriptional attenuator [ 42], up to 249 nt.
After setting a minimum protein length (100 amino acids), these orthologs were refined using the PhyloTreePruner analysis pipeline (Kocot et al. 2013).
Similar(52)
Minimum protein lengths of 76 aa represents the 5th percentile of protein lengths within the Phytozome Tester Set (below).
Predicted gene models were filtered for a minimum protein product length of 50 amino acids.
We normalized p using the signal to noise ratio of the Bernoulli distribution, which results in a normalized score, z, as follows: (1) We used aheuristic cut-off of 150 amino acids for the minimum protein or domain length to be included in our analysis.
Significant BlastP [ 108] matches were filtered using the following criteria: 80% PID over a minimum of 80% of the protein length or proteins over 100 amino acids in length, with a minimum of 75% length and 30% PID.
Significant BlastP hits were filtered using the following criteria: 80% PID over a minimum of 80% of the protein length, or proteins over 100 amino acids in length with a minimum of 50% length and 30% PID.
Related(20)
minimum gene length
minimum body length
minimum protein extent
minimum protein size
minimum seed length
minimum protein level
minimum implant length
minimum protein content
minimum protein requirement
minimum protein probability
minimum protein adsorption
minimum protein binding
minimum protein shock
minimum protein loading
minimum protein concentration
minimum protein intake
minimum protein threshold
minimum protein score
minimum protein identification
minimum description length
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