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Thus, females have a disadvantage because they will reach this presumed minimum protein threshold in a shorter period of time during catabolism.
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Note that although our method can detect shorter proteins than many other methods, we still have a detection threshold of minimum protein length.
Mass Spectra extracted from chromatogram peaks were deconvoluted using the maximum entropy setting with a mass range of 20 80 kDa, mass step of 1 Da, S/N threshold of 30, minimum consecutive charge states of 5, minimum protein fit score of 8, and an average mass of 90% peak height.
The threshold TH denotes the mean of maximum and minimum thresholds; the threshold region TR denotes the value between maximum and minimum thresholds.
Togninia minima protein sequences.
BLASTCLUST was used to apply a minimum threshold of protein sequence identity on a dataset consisting of all human Rhodopsins.
Using UniHI, users can filter interacting proteins by requiring a minimum expression threshold, and the PPI network retrieved from UniHI can be reduced to include only highly expressed proteins or extended to include lowly expressed proteins.
1,658 proteins were above the minimum abundance threshold for confident quantitation (Additional file 2: Tables S1 and S2).
Results: Given a weighted protein interaction network, our method discovers all protein sets that satisfy a user-defined minimum density threshold.
A symbol in Σ is regarded as the candidate of the next symbol if the number of its occurrences in the projected database satisfies the minimum support threshold λ and its supporting sequences include the query protein S q.
Matched peptides were further filtered at the precursor ion mass accuracy level using a 20 ppm cut-off, while protein hits were compiled and filtered using the StatQuest program with a minimum confidence threshold of 99%.
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