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This finding delineates a critical minimum protein concentration of ∼15 wt % albumin for PBSA hydrogel formation.
Approximately 2 mg of antigen at a minimum protein concentration of 0.5 mg/mL was used per rabbit.
Furthermore, the evaluation of the hydrogel crossover points between the G′ and G″ indicate a critical minimum protein concentration of 15 wt % for the formation of PBSA hydrogels.
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In a recent multivariable analysis with ICU mortality as the dependent variable [ 32], a minimum protein C concentration of less than 45% was identified as an independent risk factor in surgical ICU patients.
Therefore, to obtain the desired concentration of protein for proteomic analysis, the minimum number of samples that could give us the maximum protein concentration and sperm concentration in each group was four, and hence this was the minimum number of samples that were pooled in each group.
The minimum size of nanohydrogels depends on protein concentration and molar ratio.
The possibility of predicting the salt concentration at which minimum protein binding occurs presents new opportunities for designing better elution strategies in mixed mode protein chromatography.
Moreover, we demonstrated that very long compartments harbored a MinC protein concentration profile with multiple minima.
Alternatively, protein degradation might keep proteins at the minimum level so that a small rise in protein concentration by local translation could generate a meaningful signal.
For normalizing the sperm and protein concentration needed for proteomic analysis, a minimum total sperm concentration of about 100 X 10 sperm are required to give about 1.5 mg/mL of protein.
The OEG background kept the unspecific protein binding to a minimum, even when subjected to serum solutions with a high protein concentration.
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