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In contrast, MTX-resistant SCC-9 cells expressed minimum protein level of WWOX.
The minimum protein level of TfR1 was found at 12 h and for Fpn-1 at 24 h after rat liver irradiation).
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Additional support for this classification was inferred from an evaluation of the different minimum protein levels in commercial diets used to feed various Carnivora species maintained in captivity, including zoos and wild animal parks (Table S3).
At this minimum, protein levels are only subject to the effects of cellular dilution, which occurs at a rate μ.
Proteins were identified based on at least two unique MS/MS peptide sequences with confidence scores > 95% (corresponding to the overall minimum protein identification confidence level of 99.75%) based on Protein Pilot 3.0 peptide and protein percent confidence score criteria.
Orthologous protein-coding genes in the two succinogens were manually compiled by comparing gene order, gene orientation (forward/reverse), features of intergenic regions, and protein similarity (minimum 25% identity at the protein level).
Tracking, bleaching and image corrections are described in detail in [26] Protein accumulation onset was defined for each of the cells as the time at which protein level reached its minimum level.
The false positive rate was set to 1% at the peptide level, the false discovery rate was set to 1% at the protein level and the minimum required peptide length was set to six amino acids.
Using two doses of PFD (30 and 100 mg/kg) three times daily, PFD modulated various pulmonary cytokines at the protein level, and the minimum effective dose was found to be 30 mg/kg three times daily.
Note that novel genes were confirmed as detected and expressed at the protein level only when a minimum of two peptides mapped to that particular novel gene.
Conservatively, a gene product was confirmed expressed at the protein level only when a minimum of two unique peptides mapped to the particular gene.
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