Sentence examples for minimum protein identification from inspiring English sources

These results far exceed the minimum protein identification quality compared to other proteomic data, such as those obtained from the 2DE technique, in which only 10 to 20% of the identified proteins exhibit a coverage greater than 30% [ 14, 20].

Proteins were identified based on at least two unique MS/MS peptide sequences with confidence scores > 95% (corresponding to the overall minimum protein identification confidence level of 99.75%) based on Protein Pilot 3.0 peptide and protein percent confidence score criteria.

A newer version of Scaffold (Scaffold v4.2.0) was used and the filtering criteria were changed to: minimum of 90.0% peptide identification probability, minimum of 99.0% protein identification probability, and minimum of 2 unique peptides.

Minimum peptide and protein identification probabilities of 80 and 99% were required respectively, and a minimum of at least 2 unique peptide assignments to each identified protein.

The minima criteria for protein identification were: peptide probability <1E-04; peptide XCorr vs charge = 1.5 for charge +1; 2 for charge +2; 2.5 for charge +3; 3 for charge +3; FDR <1%.

IDPicker [109] (http://fenchurch.mc.vanderbilt.edu/) is an open source protein assembly tool that derives a minimum protein list from peptide identifications filtered to a specified FDR and increase confident peptide identifications combining multiple search engine scores.

Criteria for protein identification were: minimum of two peptides, each with a probability of >80% and an overall protein probability of >95%.

Protein identification required a minimum of 2 peptides of greater than 95% probability and a minimum of 99% protein probability.

These factors meet the criteria for protein identification that reaches a minimum MASCOT score of 35 with at least 5 matched peptides.

A protein identification was accepted if a minimum of five unique peptides matched to the protein, which corresponded to a ≥99% probability of a correct identification (see Table 1).

Protein identification was considered as unambiguous when a minimum of two peptides matched with a minimum score of 20.