Sentence examples for minimum protein loading from inspiring English sources
We also evaluated the influence of increasing hydrolysis times and solids loadings on the minimum protein loading (cellulase and β-glucosidase) required to achieve efficient hydrolysis.
The cellulase:β-glucosidase ratios were obtained by assessing the minimum protein loading required for efficient hydrolysis.
The greatest influence of hydrolysis time on the hydrolysis yields at minimum protein loading was observed with the EO pretreated samples (OPP, OPCF and OPLP).
We next wanted to confirm the role that the available surface area of cellulose might have on the minimum protein loading required for efficient hydrolysis of lignocellulosic substrates.
When the optimized minimum protein loading for efficient hydrolysis was plotted against the accessibility of the substrate, determined as the maximum amount of protein that was adsorbed (Pmax), two distinct curves were observed.
Although the cellulase:β-glucosidase ratio was optimized for minimum protein loading at a 2% solids loading, it is possible that at higher substrate concentrations, cellooligomers might be produced.
In parallel, the substrate factors (that is, the external and internal surface area of cellulose-rich substrates) that might limit the accessibility of the cellulase complex to the cellulose and the maximum protein adsorption capacity were measured for each substrate, in an attempt to correlate cellulose accessibility with the minimum protein-loading requirement for efficient hydrolysis.
Initially, two strategies were evaluated; xylanase supplementation of the minimum cellulase dose required for effective cellulose hydrolysis, and replacement of a portion of the minimum cellulase dose with xylanase while keeping the total protein loading constant.
For each mutant assayed, the internal control was used to normalize protein loading, and the experiments were performed a minimum of 2 times on two independently derived mutant isolates.
The influence of hydrolysis time and solids loading on the hydrolysis yields of the pretreated materials and on the minimum protein required to achieve efficient hydrolysis were next assessed.
GAPDH protein was used as protein loading control.
