Sentence examples for min with subsequent from inspiring English sources

Enzymes (2 μM) were incubated at different temperatures between 25 and 70°C for 20 min with subsequent cooling to 4°C in a PCR thermocycler.

Treatment of PLA films with toluene/ethanol = 3/7 mixture for 10 min with subsequent exposure in 0.001 M brilliant green dye (BGD) solution allows to entrap approximately 10− 9 modelm2 model biomolecules.

To analyze enzyme stability, enzymes (2.0 μM) were incubated at different temperatures between 25 and 70°C for 20 min with subsequent cooling to 4°C in a PCR thermocycler (Eiben et al. 2007).

SiNW-based Schottky diodes were fabricated by growing the SiNWs directly on glass substrate from 50 nm Ga at 400°C for 20 min with subsequent evaporation of both Al contacts on top of the nano-wires.

Then 120 μl of the solutions of various IgG concentrations in PBS was injected into the measuring cell, incubated for 10 min with subsequent washing of the cell with PBS until stabilization of the sensor signal.

Heat tested seeds were compared to following control sets: soaking of seeds in concentrated H2SO4 for 10 min with subsequent rinsing in running water for 3 h (Esenowo 1991), in 70 °C hot water for 40 min (Esenowo 1991), cold water for 3 days (Johansson 1999), and no treatment.

Corresponding changes in citrate were not detected in the matrix fraction, indicating citrate production in the matrix during the 20 min incubation with subsequent efflux.

VN1203ΔNS1 and VN1203ΔNS1-K58I viruses were incubated at a temperature ranging from 46°C to 58°C for 30 min with the subsequent measurement of the remaining infectious and hemagglutination titers.

The final 20 min of the recovery was used as a baseline for a stressful task: The STROOP test (STR) for 10 min with a subsequent 30-min recovery period [ 25].

For immunocytochemistry (ICC) cells were harvested by trypsinisation, washed in PBS, fixed in 4% paraformaldehyde for 25 min, stained with Meyer's haematoxylin for 5 min and centrifuged at 1500 r.p.m. for 5 min with the subsequent pellet resuspended in ice-cold 70% ethanol.

Subsequently, the sections were incubated with Equilibration Buffer for 10 min in RT, with subsequent incubation with TdT Enzyme and Reaction Buffer at 37°C for 1 h.