Sentence examples for min with cold from inspiring English sources

Cold releasing process is able to last 91 min with cold storage function.

On days 13, 14, and 15, one feed bag (containing residue) from every Rusitec fermenter was collected, washed with 100 mL of McDougall's buffer followed by a cold rinse cycle for 10 min with cold water and dried in an air forced oven for 24 h at 65 °C for further in vitro abomasum digestibility.

The cells were then rinsed twice with PS (100 mM phosphate buffer containing 0.15 M sucrose), fixed and permeabilized for 7 min with cold methanol (−20 °C), rinsed twice with PS, and then blocked for 60 min at room temperature with PS containing 0.1% BSA.

Briefly, sections were pre-treated for 15 min with cold 0.1 N HCl and 2 N HCl at 37°C for 20 min to denature DNA.

The pelleted BALF cells were treated for 10 min with cold ACK buffer to lyse red blood cells prior to analysis by flow cytometry.

Cells were washed twice with cold PBS and subsequently incubated on ice for 10 min with cold nuclei lysis buffer (50 mM Tris HCl, 10 mM EDTA, 1% SDS) to which 1 mM protease inhibitors (Sigmafast, Sigma Aldrich, St Louis, MO) and 4 mM PMSF (Sigma Aldrich, St Louis, MO) was freshly added.

Wash it after 15 mins with cold water.

Cells grown on cover slips were rinsed with PBS (phosphate-buffered saline, pH 7.4), fixed with 4% paraformaldehyde for 15 min, permeabilized with cold methanol for 10 min or with 0.5% Triton-X 100 in PBS and blocked by incubation in 5% normal horse serum in PBS containing 0.1% sodium azide (NHS/PBS-A) for at least 1 h.

Sperm, pooled from caput epididymides of three rats in each treatment group, were pelleted by cold centrifugation at 2,000 rpm for 10 min, washed with cold hyposaline for 30 min to remove traces of blood and centrifuged as before.

At different time points, the culture media was removed and cells were fixed with 4% paraformaldehyde for 15 min, washed with cold PBS and treated with 0.2% Triton-X100 for 5 min.

After 48 h, the cells were harvested, centrifuged at 800 g for 5 min, washed with cold PBS two times, centrifuged at 800 g for 5 min, and re-suspended with 70% ethanol at 4°C overnight.