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The proposed method can be completed in 30 min with low number of experimental steps.
When both two groups of endocrine cells were stimulated by glucose, the membrane particle size and Ra were higher than those not stimulated, except for IPCs that were stimulated for 30 min with low glucose concentration.
Nano-fibers were placed in a chamber of the plasma cleaner and subjected under plasma discharge for 5 min with low frequency power set as 30 W under vacuum mode.
The peptide was labeled with 18F− using unpurified cyclotron target water to obtain the labeled peptide in 23.6% ± 2.4% uncorrected yield in 35 min. The Al18F-labeled peptide had 15.7% ± 1.4% ID/g in the tumor and 79.25% ± 6.20% ID/g in the kidneys at 30 min, with low uptake in all other tissues.
The first ride is done in the rural naturalistic scenario i.e. the participant has to ride in a (virtual) rural environment for 8 10 min, with low traffic conditions; the experimenter must remember to abide by the traffic rules and not to jeopardise him/herself or the other road users during the ride.
Samples were applied to glass slides by centrifugation at 28×g for 5 min with low acceleration.
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A small falling angle of 7.5° is used to ensure long residence time in the range 3 22 min, even with low viscosity solvents such as methanol.
The method calculates time derivatives (rate of change) from successive TEC values taken from individual satellites, de-trend their variations in 15 min intervals with low order polynomial and calculates the standard deviation from residuals.
Since PMA is known to activate a variety of signalling pathways, with potentially different dose- and time-dependency, we examined the effect of PKCα and PKCδ knockdown on HB-EGF shedding after 15 and 30 min treatment with low or high concentrations of PMA.
In a separate set of experiments (21 mice) lasting 5 min, ventilation with low stretch was compared with an intermediate stretch strategy (30 ml/kg, 80 breaths/min, 3 cmH2O PEEP using O2 + 4% CO2).
To investigate the utility of AKTIV as a fluorescent probe, HeLa cervical carcinoma cells were briefly treated (5 min) with a low concentration (1 μM) of this compound.
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