Sentence examples for min with later from inspiring English sources

In response to endothelin-1, Klf2 mRNA expression was rapidly increased (∼ 9-fold; 15 30 min) with later increases in expression of Klf4 and Klf6 (∼ 5-fold; 30 60 min).

Most events (76%) resolved within 1 h (37% within 5 min), with 11% resolving later than 1 h.

First, animals were dosed with 0.05 mg/kg, about 26 min later with 0.5 mg/kg and another 26 min later with 5 mg/kg.

The mice were injected with CsA (Sandimmun, Novartis supplied as 50 mg/mL solution which was diluted 1 4 in sterile PBS and injected 100 μL at 50 mg/kg) IV by tail vein injection, or with PBS carrier, followed 30 min later with [H] MitoF (100 nmol) by IV tail vein injection and 60 min later the mice were killed and the tissues harvested.

Cryosections were fixed with 4%% paraformaldehyde in PBS for 10 min and later incubated with CAS-BLOCK (Invitrogen, CA, USA) for 1 h at room temperature.

Mice were then returned to their home cage and re-introduced in the arena 10 min later with filter papers with the odor stimuli (30 μl of male or female urine) located simultaneously in the arena at opposite corners.

On the three following nonpairing days, animals were injected with saline and 30 min later presented with the same saccharin solution for 20 min. No injections followed on these nonpairing days.

NAc glucose concentration in this case increased more slowly (∼250-s latency from the start of 120 s intragastric infusion) than after an iv injection, but the increase was much larger (∼750 μM, or doubling of its levels) and with later peak (20 30 min).

We harnessed honeybees in individual metal holders and after two and half hours of rest, we first measured US responsiveness by touching the antennae with water (control) and then, 15 min later, with the US.

Tissue sections were then digested with 0.1 mg/mL proteinase K (Roche Diagnostics, Indianapolis, IN) in 0.6 M Tris (pH 7.5)/ 0.1% CaCl2 (proteinase K buffer) for 15 min and later blocked with 20% normal sheep serum in Tris-saline-tween-20. Tissue sections were incubated for 60 min with a primary antibody.

The cells were fixed 30 min later with 4% PFA, stained with Hoechst33258 (50 µg mL−1, Sigma-Aldrich; Inc) and mounted in Mowiol 4 88 (Calbiochem).