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Discharge operations can be up to 30 min with full heating power.

Cells were incubated for 5 min with full DMEM containing 30 mM MES (pH 4.5) or full DMEM (pH 7.4) at 37°C.

Hemodynamic and respiratory variables are shown in Table  1 for the two groups of animals, treated with NS or OA and euthanized after 1 h (Table  1).> -wrap-foot> * =  p < 0.01 Each assessment of B-lines was performed in less than 5 min, with full feasibility of 100%%.

After each session subjects had a 5-min break with full daylight.

These steps were followed by 15 min centrifugation at 4 °C with full speed, during which the mixture separated into different phases, with the upper phase containing the RNA.

The kinetics of catalytic reaction and of catalyst deactivation have been studied by conducting a series of consecutive tests at increasing temperatures in steps of 20 °C and monitoring the evolution of the reaction for periods of 30 min on stream at each temperature, with full product analysis every 3 min.

High-quality data were obtained for the enzymatic hydrolysis of 4-nitrophenyl glucopyranoside by sweet almond β-glucosidase: time-resolved kinetics for individual droplets with different substrate concentrations were used to derive kcat and KM values within 20 min in a single run, with full automation from droplet generation to data collection.

To stain mitochondria (MT), cells were incubated with 250 nM Mitotracker Red in pre-warmed full medium for 30 min at 37°C and washed twice with full medium.

Rising luminescence was observed when worms were removed from azide, with full recovery within 30 min, as established by comparison with controls not exposed to NaN3.

To stain the mitochondria (MT), cells were incubated with 250 nM Mitotracker Red in pre-warmed full medium for 30 min at 37°C and then washed twice with full medium.

We visited each station on average 7 times for approximately 19 min with two teams carrying a full set of instruments.