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The neutron signal continued for more than 20 min with high statistical significance.
18F-FBA was synthesized within 35 min with high RCY (70 84%, d.c).
The small amount of proposed adsorbent (0.2 g) was applicable for removal of MB (RE > 95%) at 16 min with high adsorption capacity (82.9 mg g−1).
The other advantages are portability, ease of implementation and rapid analysis (3 min) with high precision (RSD ≤ 2.5%) and high accuracy (Recovery = 98.7% ± 1.6).
The small amount of adsorbent (0.008 0.015 g) is applicable for successful removal of JGB (RE > 99%) in short time (7 min) with high adsorption capacity (81.3 98.03 mg g−1).
Here we report the design of a bioluminescence resonance energy transfer (BRET -based sensing system that could detect nucleic acid target in 5 min with high sensitivity and selectivity.
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However, sonication for 5 min with high-power-ultrasound (18 kHz and 40 kHz) or megasonics (MS) (2 MHz) (80 90 kJ kg−1) increased G′ and G″.
After centrifugation, the nuclear pellet was vortexed for 20 min with high-salt extraction buffer (50 m M HEPES, 400 m M KCl, 1 m M EDTA, 1 m M EGTA, 1 m M DTT, and protease and phosphatase inhibitors, pH 7.9).
With the visual observations mixing times varied between 4 s with low volume injection and 900 rpm shaking frequency, and 3 min with higher volume injection and no shaking.
Within the ovarian cortex, the lowest level of LDH release, and therefore cellular damage, was detected following the shortest periods of CPA perfusion (3 and 10 min), with higher levels at 15 and 30 min (P < 0.05) and with 60 min (our standard perfusion time) being intermediate between these two extremes.
Immune complexes were collected with ss protein A (45 min) and washed three times (5 min each) with high salt buffer (washing buffer: 20 mM Tris, pH 8.0, 0.1% SDS, 1% NP-40, 2 mM EDTA, 500 mM NaCl) and three times with low salt buffer (1× Tris/EDTA [TE]).
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