Sentence examples for min before quenching from inspiring English sources

The products were identified by reference to a Maxam-Gilbert C+T sequencing ladder generated by treating the substrate with 60% hydrazine for 10 min before quenching as described above.

The solution was stirred for 15 min before quenching with Na2S2O5 (aq. saturated).

At TUNEL staining, sections were treated with proteinase K for 15 min before quenching endogenous peroxidase activity.

The reaction was allowed to occur at room temperature for 30 min before quenching with 100 mM Tris, pH 8.0.

Sections were denatured in 70% formamide (in 2 × SSC, pH 7.0) at 75°C for 2 min before quenching in ice-cold ethanol.

Labeling reactions were carried out in the dark on ice for 30 min before quenching with 1 mL 10 mM lysine for 10 min on ice.

Color was developed for approximately 30 min at 37 °C before quenching with 50 μL of 2 M sodium hydroxide.

Reactions were initiated by mixing 1 µM ternary complex with 0.1 µM 70S IC and incubated for 1 min at 21°C before quenching with 1/5th volume of 2 M KOH and 1 M H2O2.

DNA protein interactions were cross-linked using 1% formaldehyde for 10 min at room temperature, before quenching with a final concentration of 125 mM glycine.

Cells were fixed in 1% molecular biology grade formaldehyde (Sigma) 9 min before being quenched with glycine (0.125 M final concentration).

Reactions were incubated at 50°C for 15 min before being quenched by the addition of 20 μl loading dye (8 M urea, 1 × TBE, 0.05% Bromophenol Blue) and incubated for 5 min at 99°C.