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A bacitracin disc was placed in the selective culture broth for 15 min before sampling.
10 g/L pyrite was added to a 1.5 mg/L RNA solution, stirred and centrifuged (4°C, 4500 × g, 5 min) before sampling.
Water was collected from open wells using drawing buckets tied with ropes, while boreholes were pumped for 5 15 min before sampling.
Water was collected from open wells using drawing buckets tied with ropes, while bore wells and tube wells were pumped for 5 15 min before sampling (Andrew et al. 2011).
Tooth brushing, smoking, and food and drink intake were to be avoided 30 min before sampling.
The ICG was infused continuously for at least 90 min before sampling to achieve steady state conditions of ICG concentration.
Similar(48)
The supernatant was heated at 70°C in 15 µl SDS-PAGE loading buffer for 10 min before samples were run in NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen) and transferred to an iBlot membrane (Invitrogen) for western blot analysis.
Protein-bound 125I (125I-PEGLA or 125I-PEGBSA) in blood was measured by mixing 100 µl blood with 1 ml of 20% (w/v) trichloroacetic acid (TCA, Sigma) on ice for 5 min before samples were spun at 8,000× g for 1 min. TCA-precipitated 125I was defined as protein-bound 125I whereas non-precipitable 125I was considered free-iodine.
with the patients in the upright position for at least 30 min before sample collection.
Propidium iodide was added 30 min before sample analysis, and data were collected as.fcs files.
Blood sampling for ARR was always performed in the morning hours (0700 1000 h) with the patients in the upright position for at least 30 min before sample collection.
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