Sentence examples for min before application from inspiring English sources

Throughout the experiment, the rate of impulses per second was visualized and recorded for a control period of at least 20 min before application of substances.

The antagonist was injected intravenously in 0.03 mg/kg (black bars), 0.3 mg/kg (light grey bars) and 3 mg/kg (dark grey bars) over a period of 20 min before application of IS. c The TRPV1 antagonist JNJ-17203212 was injected in 0.3 mg/kg (black bars), 3 mg/kg (light grey bars) and 30 mg/kg (dark grey bars) previous to IS administration.

Preincubation of neurons with 50 µM PTX for 30 min before application of the dye further reduced FM1-43FX uptake in CHL1−/− but not in CHL1+/+ synaptic boutons (Fig. 10B).

Probes (4 ng/µL) were combined in hybridization buffer supplied with the dyes, denatured for 10 min at 72°C in 50% formamide in 2X SSC, and kept on ice for 30 min before application to the slides.

Sections were then incubated with 2% BSA/TBS for 30 min before application of primary antibody.

Equal amounts of protein (10  μg) were loaded on 8% SDS polyacrylamide gel and boiled 3 min before application.

The MTV was measured for 75 min. The MTV results were calculated by the following equation: Vt/V0 × 100%%, where Vt is the average velocity in each 5 min interval and V0 the average velocity in the last 5 min interval before application of the test solution.

We allowed at least 30 min washout before application of subsequent drugs.

Thereafter, platelet SDS lysates were pooled with 10 µl of RIPA-PPP fraction, with addition of 70 µl 2%% BSA/PBS buffer to bind excess SDS, and incubated (25 min, 4 °C) before application onto the protein biochip.

The next day, sections were washed 3 times in PBS (5 min each), before application of a species specific secondary antibody (Molecular Probes) for 1 hr in 0.1%–0.3% triton/PBS.

The frequency of [Ca2+]i oscillations was calculated during a period of 2 4 min before stimuli application (control), and the frequency was measured during 5 min of stimuli application calculated over the period from minute 5 to minute 10 after stimuli were applied.